| Parameters | Method/Norms |
| Appearance | Brown color viscosity syrup with characteristic odor. Determination was conducted visually. |
| PH | 3.0 - 6.0 |
| Density | 1.25 to 1.35 g/cm3 |
| Originality | |
| Polysaccharides | Qualitative reactions with: With of 96% spirit Should be precipitate suspension was formed |
| Tanning agents | With of Ferric chloride solution 3% Should be appears of greenish-yellow coloring |
| Ascorbic acid | With of spirit solution of sodium 2.6-dichlorfenolindofenolite Appeared reddish-violet coloring |
| Alkaloid vasicine determination | TLC, 1 reddish pink color spot (vasicine) was shown on the plate after treatment with Dragendorff reagent in UV light at wave length 254 nm |
| Methylparahydroxybenzoat Propylparahydroxybenzoat | HPLC, Periods of 2 main peaks retention should match to peaks retention periods of on standard solutions |
| Quantitative determination | |
| Total alkaloids | Spectrophotometric determination , Total alkaloids calculated as vasicine should not be less than 0.035% and not more than 0.07% |
| Methylparahydroxybenzoat Propylparahydroxybenzoat | HPLC, Methylparahydroxybenzoate 9.0 - 11.0 mg/10 ml of preparation Propylparahydroxybenzoate 1.8 - 2.2 mg/10 ml of preparation |